||PI: Deyou Zheng, Ph.D.
Email: deyou.zheng aT einstein.yu.edu
Office: Price 320
Our lab is in the Department of Neurology at Albert Einstein College of Medicine of Yeshiva University. We are located in Rose F. Kennedy Center for Intellectual and Developmental Disabilities Research. Our lab is also affiliated with the Institute for Brain Disorders and Neural Regeneration, and member of the Department of Genetics and the Department of Neuroscience. The research areas of the lab are computational genomics and bioinformatics, with a strong focus on mining large-scale genomic data. We develop and apply computational techniques for integrating data from comparative genomics and functional (epi)genomics to understand the structure, function and evolution of the human genome, and to investigate its functional importances in developments and diseases.
Decoding the human genome and others closely related to it is our primary research interest, and comparative genomics and integrated analysis of functional genomics data are our major research approaches. In general, we are interested in developing and applying computational methods for exploiting patterns in high-throughput genomic data and for integrating information from experimental and computational biology. We are highly interested in studying the expression, regulation, and evolution of human nervous systems genes (coding or noncoding), especially those implicated in neurodegenerative diseases, using experimental data from next-generation sequencing (e.g., ChIP-Seq and RNA-Seq). Specifically, our research group will be focused on these areas:
+ Gene duplication, to investigate how gene duplication has shaped the human genome and how novel genes or regulatory elements emerge in humans and other primates.
Gene expansion (through either duplication or retrotransposition) is a major driving force for the emergence of novel functions during evolution. Inter- and intra-genome sequence comparison can reveal DNA elements that are either uniquely present or specifically selected in certain species. Tracking the evolutionary history of such sequences can lead to the discovery of genes or regulatory elements (including non-coding RNAs) that function specifically in humans. Thus, the long-term goal is to search for functional genomic components that set us apart from other animals. To this end, our research will compare the human genome with other mammalian (e.g., chimp, macaque, dog, and mouse) genomes to exploit the role of gene duplication in generating novel protein-protein interactions and novel biochemical pathways. We are interested in both the birth and the death (pseudogenization or loss) of such lineage-specific functional DNA sequences, especially for those involved in the development of nervous system and brains.
+ Integrated analysis of functional genomics data, to explore computational techniques for combining experimental and computational genomics data in order to achieve a global understanding of the function of the human genome.
We are interested in developing bioinformatics algorithms to conduct cross-genome sequence comparisons (e.g., ortholog assignment) with a focus on aims of our specific research. As large amount of functional genomic data from diverse sources (microarray, high-throughput sequencing, protein-protein interaction, etc.) will be used in our studies, our group plan to develop effective computational methods for data integration and at the same time for addressing common concerns of data quality in genome-scale experiments. A rigorous statistical framework needs to be built in order to extract biologically meaningful signals from noises or stochastic background.
+ Functional implication of alternative splicing, to investigate how higher eukaryotes (e.g., humans) achieve functional diversity through producing multiple RNA transcripts from one gene locus.
We are particularly interested in the generation of isoforms in different tissues / cells and how these special isoforms carry out their tissue-specific functions.
- YALE UNIVERSITY, New Haven, CT. 2003-2007, Postdoctoral Fellow, Department of Molecular Biophysics and Biochemistry. Research Area: Bioinformatics & Human Genomics.
- RUTGERS UNIVERSITY, Piscataway, NJ. Ph.D. in Biochemistry, 2003. Rsearch Area: Structural Genomics & Bioinformatics.
- AUBURN UNIVERSITY, Auburn, AL. M.S. in Pathobiology, 1998. Research Area: Molecular Biology.
- BEIJING AGRICULTURAL UNIVERSITY, Beijing, China. B.S. in Microbiology, 1992
+ Xingyi Guo, Postdoctoral Fellow. 09/08-03/13
We are looking for enthusiastic undergraduate students, graduate students, and postdoctoral associates to join our group. Reasonable computer programming skills is a plus. Candidates are expected to work in a wide range of bioinformatics projects. Please contact the PI, Deyou Zheng, for details.
Selected Publications (*co-corresponding authors)
1. Wang P#, Lin M#, Pedrosa E, Hrabovsky A, Zhang Z, Guo W, Lachman HM*, Zheng D*. (2015). CRISPR/Cas9-mediated heterozygous knockout of the autism gene CHD8 and characterization of its transcriptional networks in neurodevelopment. Molecular Autism 6:55. (*co-corresponding authors)
2. Adam RC, Yang H, Rockowitz S, Larsen SB, Nikolova M, Oristian DS, Polak L, Kadaja M, Asare A, Zheng D, Fuchs E. (2015). Pioneer factors govern super-enhancer dynamics in stem cell plasticity and lineage choice. Nature 521:366-370
3. Rockowitz S, Lien WH, Pedrosa E, Wei G, Lin M, Zhao K, Lachman HM, Fuchs E, Zheng D. (2014). Comparison of REST Cistromes across Human Cell Types Reveals Common and Context-Specific Functions. PLoS Comput Biol 10: e1003671
4. Chen Y, Chi P, Rockowitz S, Iaquinta PJ, Shamu T, Shukla S, Gao D, Sirota I, Carver BS, Wongvipat J, Scher HI, Zheng D, Sawyers CL. (2013) ETS Factors Reprogram the Androgen Receptor Cistrome and Prime Prostate Tumorigenesis in Response to PTEN Loss. Nat Med 19: 1023-1029.
5. Wontakal SN, Guo X, Smith C, Maccarthy T, Bresnick EH, Bergman A, Snyder MP, Weissman SM*, Zheng D*, Skoultchi AI*. (2012). A Core Erythroid Transcriptional Network is Repressed by a Master Regulator of Myelo-lymphoid Differentiation. Proc Natl Acad Sci USA 109: 3832-3837
6. Goldberg AD, Banaszynski LA, Noh KM, Lewis PW, Elsaesser SJ, Stadler S, Dewell S, Law M, Guo X, Li X, Wen D, Chapgier A, DeKelver RC, Miller JC, Lee YL, Boydston EA, Holmes MC, Gregory PD, Greally JM, Rafii S, Yang C, Scambler PJ, Garrick D, Gibbons R, Higgs DR, Cristea IM, Urnov FD, Zheng D*, Allis CD* (2010). Distinct Factors Control Histone Variant H3.3 Localization at Specific Genomic Regions. Cell 140:678-691.
7. Guo X, Zhang Z, Gerstein M, Zheng D. (2009) Small RNAs Originated from Pseudogenes: cis- or trans-Acting? PLoS Comput Biol 5:e1000449.
8. Zheng D*, Zhao K, Mehler M. (2009) Profiling RE1/REST-mediated Histone Modifications in the Human Genome. Genome Biol 10:R9. (*corresponding author)
9. Zheng D. (2008) Asymmetric Histone Modifications between the Original and Derived Loci of Human Segmental Duplications. Genome Biol 9:R105.
10. Zheng D, Gerstein M. (2007) The Ambiguous Boundary between Genes and Pseudogenes: the dead rise up, or do they? Trends Genet 23: 219-24.